Abstract: Objective: To search, evaluate and integrate best practice evidence of blood culture collection in children with blood stream infection. Methods: According to the 6s model, Guidelines were searched in following databases: UpToDate, NICE Guidance, the Scottish court guidelines network (SIGN), World Health Organization (WHO), registered nurses association of Ontario (RNAO), international guidelines for the network (GIN), the national guide Library (NGC), medlive, the clinical and laboratory standards institute (CLSI), Pubmed, CINAHL, the Cochrane Library, CNKI, Wanfang Data, Sinomed. The latest systematic reviews were supplemented. Two researchers evaluated the quality of the included literature, extracted and summarized the evidence from the literatures. Results: A total of 7 articles were included, including 1 clinical decision, 4 guidelines, 2 systematic reviews. Totally 22 pieces of evidence on 6 aspects including blood collection time, disinfection, collection tools, blood collection method, blood collection volume, management strategy were finally summarized. Conclusion: The summarized evidence of blood culture collection of children can be used in clinical practice to promote the standardized management of blood culture collection of children and improve the detection rate.

Key words: children; bloodstream infection; blood culture; best practice evidence; detection rate