Abstract: Seamless integration of multidisciplinary practice that includes providing a good linkage among hospital,care providers, patients, and family is an essential way to enhance the well-being of children and their family during the pediatric hematology and oncology care phases. This study introduces the experience and practice of the total cycle of care for children with leukemia. The total care cycle penetrates the whole process of diagnosis, hospitalization, discharge preparation,and post-discharge care continuing process. It utilizes the hospital health informatics platform to assess and evaluate children and family’s needs in each treatment phase; this care model also improves cooperation among multidiscipline, doctors,nurses, and families. Finally, this total care cycle has achieved the optimal care outcome for children and families during the cancer therapy. This total care cycle approach could help future pediatric hematology and oncology nursing practice.