Abstract: Objective: To evaluate the quality of the clinical practice guidelines related to breastfeeding in hospitalized neonates by using the Appraisal of Guidelines for Research and Evaluation II (AGREE II) instrument, and to provide reference for the development of breastfeeding guideline in China. Methods: The clinical guidelines for breastfeeding were identified and accessed by searching PUBMED database, WANFANG database, CNKI database, NGC, NICE etc.AGREE instrument was used to evaluate the quality of the latest versions of the clinical guidelines. Results: Ten guidelines were enrolled, and all were in English. Among them, 4 were breastmilk bank operation guidelines, and 8 were evidencebased guidelines. The results showed that the average scores in the 6 fields were 88.8%, 54.6%, 65.2%, 85.0%, 48.92% and 40%, respectively. Eight guidelines were positively recommended, and 2 were uncertain. Conclusion: The quality of the 10 clinical practice guidelines related to breastfeeding in hospitalized neonates was relatively high. But in the aspects of rigor in guideline development, applicability and independence, the guidelines need to be improved. The guidelines positively recommended could give good evidence and reference to promote breastfeeding in China. However, guidelines should be used according to our clinical situation, and local clinical practice guideline should be developed.