Abstract: Objective: To explore the application of comprehensive simulation method in experimental teaching of midwifery nursing. Methods: Totally 99 undergraduate students from grade 2005-2008 in Nursing Department of Tianjin Medical University were included in this study, they were divided into experimental group (n=49, Grade 2007 and 2008) and control group (n=50, grade 2005 and 2006). The comprehensive simulation experiment teaching method was implemented in experimental group, while traditional teaching method was used in control group. A self-designed satisfaction questionnaire was used to evaluate the students' satisfaction status on this comprehensive simulation teaching method. The course scores between the two groups were also compared. Results: The course score of midwifery nursing in experimental group was higher than control group (P<0.05). They reported that this comprehensive simulation experiment teaching method improved their nursing professional skills and problem-solving ability, which was good for clinical practice. Conclusion: Comprehensive simulation experiment teaching may improve the nursing professional skills and comprehensive ability of students.