Abstract: In order to improve the emergency operation cooperation quality in large hospital and ensure the operation safety, we organized the related experts to complile the electronic operation cooperation information booklets in our hospital according to the actual situation and informationization development trend of emergency operation room. We investigaed the doctor and nurses’ satisfaction, charging accuracy, the mean preparing operation time, and the preparing operative materials rates of reaching the standard,through questionnaires and follow-up before and after the application of electronic operation cooperation information booklet. The results showed that the application of electronic operation cooperation information booklet could significantly improve the emergency operation cooperation quality and the satisfactions of doctors and nurses. This would be a very good reference for other hospitals.