Abstract: Objective: To investigate the impact of nurses' perceived organizational support and job satisfaction on their turnover intention, and to establish a model of this relationship. Methods: Totally 592 nurses from a 3-A grade hospital were investigated with Survey of Nurse Perceived Organizational Support (SNPOS), McCloskey/Mueller Satisfaction Scale (MMSS)and Turnover Intention Scale. The data were analyzed by SPSS 17.0 and AMOS 17.0. Results: The mean scores of SNPOS,MMSS, Turnover Intention Scale were 3.23±0.76, 3.06±0.56, and 12.87±3.77, respectively. Perceived organizational support had positive correlation with job satisfaction and negative correlation with turnover intention. The job satisfaction had negative correlation with turnover intention. The job satisfaction mediated between nurse perceived organizational support and turnover intention, with the mediation effect of -0.224, and explained 43.6% of the total effects. Conclusion: Nursing administrators should provide favorable organizational support to nurses in order to enhance their job satisfaction and decrease their turnover intention.