Abstract: Objective: To explore the job stressors and burnout level among nurses with different educational levels in clinical units of tertiary hospital. Methods: Totally 1550 nurses in three general hospitals were investigated with Maslach Burnout Inventory(MBI) and Nurse Job Stressors Scale (NJSS). Results: The average score of nurse burnout is 70.64±18.15, and the score of every dimension was significantly higher than the Chinese norm of nurse. The scores of stressors were different among the nurses with different educational levels (P<0.01). The nurses with Bachelor degree had the highest NJSS score, while the graduate nurses had the lowest. Conclusion: The burnout of nurses was in a high level. Interventions should be implemented to cope with these different stressors in different nurses with educational backgrounds.