Abstract: Objective: To evaluate the validity and reliability of an organizational citizenship behavior (OCB) scale for nurses in China. Methods: Totally 317 nurses were recruited by purposive sampling method and investigated by nurses’ OCB scale designed by the authors during the early study and 34 nurses were involved in a 2-week test-retest survey. Exploratory factor analysis, internal consistency, and test-retest reliability analysis were conducted. Results: Through 9 times of exploratory factor analysis, 8 items were deleted and 24 items were reserved. We extracted 5 factors which were self-development, responsibility consciousness, actively serving, helping colleagues, and organizational identity. The cumulative variance explanation rate of these 5 factors was 64.14%, and each item’s loading value ranged from 0.513 to 0.814. The Cronbach's a coefficients of the overall scale and 5 factors were 0.934, 0.897, 0.813, 0.802, 0.813, and 0.809, respectively. The test-retest coefficient was 0.74 (P<0.001).Conclusion: The 24-item OCB scale for nurses based on 9 times of exploratory factor analysis has good construct validity,internal consistency and test-retest stability.