Abstract: Objective: To explore the accuracy of chemical indicator in pressure steam sterilizing package on monitoring of sterilization effect in sterilization process. Methods: In accordance with the original implementation of Industry Standards of Disinfection and supply Center issued by Ministry of Health on December 1, 2009, we strengthened the management of the whole process of sterilization using the questionnaire survey method, experimental method and random sampling method.We compared the monitoring effect of chemical indicator in sterilization process of different manufacturers commonly used in Chinese hospitals. At the same time, we analyzed the causes of nonconformities,implemented the improvement measures,and improved the package chemical indicator's accuracy of monitoring sterilization effect. Results: The effect of sterilization monitoring were associated with production manufacturer defect, packaging materials with poor permeability, direct contact of chemical indicator with the metal utensils, too large package, too full loading before sterilization, unsatisfactory steam quality,sterilizer operation mistake, and failure operation. Conclusion: The sterilization effects of package chemical indicator were influenced by many factors. The managers should take the initiative to strengthen the sterilization process details management,properly select qualified package chemical indicator according to the different risks level, structure of operation objects, different material, and provide accurate and valuable monitoring results for sterilization package to ensure the quality of sterile objects,medical safety, and a comprehensive high quality nursing service.