Abstract: Objective: To implement theoretical practice all-in-one teaching in curriculum integration of medical and surgical nursing and explore the influence of the implementation on educational environment. Methods: The theoretical practice all-in-one teaching method was designed and implemented through the combination of students' autonomous exploration, group discussion and clinical scene learning under the background of curriculum integration of medical and surgical nursing. From teachers and students investigation, education environments of traditional teaching and theoretical practice all-in-one teaching were evaluated.Results: The education environment of theoretical practice all-in-one teaching got high scores from both the teachers and students(3.15±0.13 & 3.04±0.33, respectively). There were significant differences between the new teaching method and the traditional one (P<0.01).Conclusions: Theoretical practice all-in-one teaching could be an effective method in this context of curriculum integration. But it still needed deep exploration on mobilization, professional emotional education, communication skills training and so on.