Abstract: Objective: To explore the application of Problem-Based Learning (PBL) teaching model in emergency room among nursing undergraduate interns. Methods: Totally 72 nursing undergraduate interns in the emergency room were recruited and randomly divided into control group (n=36) and experimental group (n=36).The control group were taught by Lecture-based learning (LBL) approach, while the experimental group used the PBL teaching method. We compared the theory examination scores and comprehensive examination skills scores of the students in two groups, and investigated the students' recognition to PBL teaching method and their satisfaction. Results: The theory test and comprehensive skills assessment scores among students in the experimental group were significantly better than those in the control group (P<0.05). The students in the experimental group had high subjective recognition and willing to participate in PBL teaching. Conclusion: The application of PBL teaching model in emergency room among nursing undergraduate interns could improve the students' clinical comprehensive ability,motivate their learning interest, and cultivate their autonomous learning ability.