Abstract: Objective: Relying on “Positional Duel Education Mode” with schools and hospitals cooperation, we constructed nursing professional course to achieve the comprehensive combination of the nursing professional course and the occupational standard. Methods: On the basis of hospital works, the content of the course was restructured,and specific professional courses were designed to make the students successful in the practical setting. Results: Students survey showed that teachers rating score vary from (7.92±1.43) to (8.46±1.36) for their classroom teaching means and methods, the teaching effect, practical level, and advanced knowledge. Conclusions: “Positional Duel Education Mode” is career-oriented curriculum system closely combined with professional standards which benefits the students, schools and hospitals. Also, it motivates and proactive of three parties and improve the teaching outcomes and quality of professionals in the practical setting.