Abstract: Objective: To investigate the knowledge status of occupational protection for blood-transmitted disease in logistics staff from a primary hospital. Methods: The authors investigated and analyzed the knowledge of the protection measures against occupational exposure to blood-transmitted diseases among the logistics staff of their hospital through a questionnaire survey.Results: Only 27.32% of all respondents acquired protection knowledge through training organized by the hospital; in terms of selfprotection attitudes, 53.61% of all respondents paid attention but lacked of action, 11.86% had no awareness and took no protection measure; 31.44% had not been vaccinated with Hepatitis B vaccine; the rate of correct answers to relevant questions on bloodtransmitted diseases was only 57.84%. Conclusion: Hospital administrators should pay attention to and enhance the education and management of front-line logistics staff's knowledge of blood-transmitted diseases, improve their compliance on self-protection,and timely vaccinate them, in order to effectively prevent or reduce the possibility of infection of blood-transmitted diseases.