Abstract: Objective: To optimize hospitalized treatment process of intravenous thrombolysis among patients with ischemic stroke, to shorten Door-to-Needle Time (DNT), and to improve the thrombolysis rate within the hospitalized time window of intravenous thrombolysis. Methods: We established a "parallel treatment" model for hospitalized treatment process of intravenous thrombolysis and used the observational and quasi-experimental research methods to compare the time from the hospital to medication (DNT), the rate of thrombolysis within the hospitalized time window, the time of key links and clinical outcomes before and after process optimizing. Results: After the process optimizing, the median DNT was 62 minutes which was shorter than that before the process optimizing (P<0.01), and the thrombolysis rate increased by 76.3% within the time window. The median time from nurse admissions to the emergency doctors admissions was shortened 3 minutes (P<0.01), and the median time from nurse admissions to thrombolysis doctors admissions was shortened 9 minutes (P<0.01). The median time from nurse taking a blood sample to test outcomes returning was shortened 23 minutes (P<0.01), and the median time from nurses admissions to CT completing was shorten 13 minutes (P<0.01). The median time of family decision time was shortened 12 minutes (P<0.01). The median time of hospital stays was shortened 2 days (P<0.01). Conclusion: The effect of hospitalized optimized treatment process of intravenous thrombolysis is obvious, and DNT time, the time of key links, and the thrombolysis rate within the hospitalized time window has been improved, and the prognosis of patients have been improved.