Abstract: Objective: To explore the relationship between nurse-nurse collaboration and the subjective wellbeing of nurses, and to provide reference for nursing managers to enhance the subjective well-being of nurses from the perspective of nurse-nurse collaboration. Methods: Totally 340 nurses from 3 tertiary hospitals in Heifei were recruited and investigated by the demographic questionnaire, Nurse-Nurse Collaboration Scale, and Campbell Index of Well-being.Results: The mean score of nurse-nurse cooperation score of was (80.75±8.90), among which the specialization dimension got the highest score (3.33±0.44), and the communication dimension got the lowest score (3.13±0.47). There was significant positive correlation between nurse-nurse cooperation and nurses' subjective well being (P<0.05). The influencing factors of the subjective well-being of nurses were different working departments, professional dimension and sharing dimension of nurse-nurse collaboration (P<0.05). Conclusion: The higher the level of nurse-nurse collaboration is, the higher subjective well being is. Nursing managers should focus on the professional ability and the sharing process of nurse-nurse collaboration, develop effective strategy to improve nurses' subjective well-being, and pay more attention to improve the department working environment.