Abstract: Objective: To investigate the relationship between subjective well-being and job burnout among clinical nurses,and the role of empathy. Methods: A convenience sample of 231 registered nurses was investigated with demographic data, Index of well-being, Interpersonal Reactivity Index-C and Maslach Burnout Inventory. Results: The average score of well-being was 11.54±1.97. The average score of emotional exhaustion, depersonalization and reduced personal accomplishment were 16.21±9.78,4.20±4.30 and 17.45±10.85. The average score of empathy was 53.33±9.41, and the moderate effect of empathy and its dimension were significant. Conclusion: The present data suggest that the level of empathy and its dimension of imagination, sympathy moderate the negative effects of job burnout on subjective well-being among clinical nurses, while personal suffering encourages the negative effects.