Abstract: The management of environmental surface cleaning is the basis for prevention and control of healthcare associated infection (HAIs) among the healthcare facilities. The contaminated surface is a primary source for susceptible patients to acquire pathogens. Improving the quality of environmental surface cleaning can effectively decrease the outbreak of HAIs. Enhanced terminal cleaning and disinfection can reduce 10% to 30% of HAIs caused by multidrug-resistant organism or Clostridium difficile. Inappropriate cleaning and disinfection cannot remove the surface contamination from hospital environment and even may cause pathogens spreading within hospitals. National Health and Family Planning Commission of the PRC issued "Regulation for cleaning and disinfection management of environmental surface in healthcare facilities" in the end of 2016. This paper explains the importance of environmental cleaning for clinical nursing, including the requirement of management, principles of cleaning and disinfection, daily cleaning and disinfection, enhanced cleaning and disinfection, reusing of cleaning products. The implementation and compliance with this regulation will provide a clean and safe environment for the patients.