Abstract: Objective: To evaluate the clinical effectiveness for patients and benefits for medical personnel of pre-filled saline syringes compared with manual operation. Methods: We searched public literatures in Pubmed, the Cochrane Library, Embase, CNKI, WanFang Data and VIP databases for the period up to March 2015. The search terms used were: flush, pre-filled saline syringes and pre-loaded syringes. RevMan 5.3 Software was used to conduct the review after the quality assessment of included studies. Results: In total, 46 studies were included for a further analysis. As for patients' benefits, Meta-analysis results showed significant greater effects of pre-filled saline syringes versus manual operation in reducing catheter-related blood stream infections (odds ration [OR] 0.23, 95% CI : 0.16, 0.34, P<0.001), occlusions (OR 0.38, 95% CI : 0.24, 0.60, P<0.001), and extending catheter indwell time (Mean difference [MD] 0.51d, 95% CI : 0.34, 0.67, P<0.001). As for medical personnel's benefits, this analysis also represented evidence of pre-filled saline syringes in reducing the rate of needlestick injury (OR 0.21, 95% CI : 0.07, 0.61, P=0.004) and medical personnel's operation time (MD -1.37min, 95% CI : -1.56, -1.18, P<0.001) when compared with manual operation. Conclusion: Compared with manual operation, pre-filled saline syringe is a better option for patients to reduce the rate of catheter-related bloodstream infection, occlusions and extend catheter indwell time, and for health workers to reduce the rate of needlestick injury and operation time as well. It's meaningful to apply pre-filled saline syringes in clinic.