Abstract: Objective: To investigate and understand job tasks of nurses with different professional titles in hospitals of various levels in China, so as to provide references for the establishment of nursing qualification examination system matching with the clinical requirements. Methods: Typical layered sampling method was adopted to select proper samples in 7 geographical zones and 3 types of medical institutions. We used the "Questionnaire of Job Tasks of Nurses with Different Professional Titles" to investigate 9 034 nurses in these selected medical institutions. Results: The average score of the item "nurses' task execution frequency" was (3.09±0.71) points, and the average score of importance evaluation was (3.35±0.50) points. The correlation analysis of execution frequency and importance evaluation of each dimension showed a significant positive correlation (P<0.01). Variance analysis showed that task execution frequence of nurses with different titles and the importance evaluation of each dimension was significantly different (P<0.05), while tasks execution frequency of nurses in hospitals of various levels and the importance evaluation of part dimensions were significantly different (P<0.05). Conclusion: Professional title and hospital level had an influence on tasks execution frequency of nurses and the importance evaluation, so the nurse professional setting and the advanced examination reform should take these two factors, i.e. hospital level and nurses' professional titles into serious consideration.