Abstract: Objective: To establish a qualification and evaluation system for clinical instructors for Master of Nurisng Specialist, and provide effective basis for the selection, training, and evaluation of the instructors. Methods: Through literature review, open questionnaire survey, and experts interviews to set the preliminary index system. The Delphi method was used for two rounds of 33 experts' consultations. Result: In the two rounds of consultations, experts' positive coefficients were 87.9% and 89.7%, respectively. The authoritative coefficients of experts were 0.853 and 0.872, respectively.The Kendall's W coefficients were 0.231and 0.295, both of them were statistical significance (P<0.01). The evaluation system included 9 access indicators, 5 dimensions, 19 evaluation indicators, and 56 evaluation items. Conclusion: The whole system for clinical instructors of Master of Nursing Specialist is highly scientific, which is able to serve as an effective tool to manage clinic instructors.