Abstract: Objective: To investigate the cleanness of mobile phones in medical staff using ATP bioluminescence assay, and explore the effect of alcohol and wipes to clean and disinfect the cell phone. Methods: One hundred and eighty medical staffs were selected by stratified randomized sampling method. Their mobile phones' cleanliness was detected by ATP bioluminescence assay.After the detection, the investigator randomly cleaned 30 mobile phones with 75% alcohol, comparing the effect of disinfection after 5 minutes, 10 minutes and 20 minutes. They also randomly selected 60 mobile phones to compare the effect of cleaning and disinfection with general wipes and sanitary wipes. Results: The cleaning percentage of mobile phones of medical staffs was 5.56%.The ATP values of cell phones in special sections (such as Intensive Care Unit, Department of Infectious Diseases, and Blood Purification Center) were significantly lower than that in department of surgical and medical (P<0.05). Hands washing before using the phones or protecting them with a disposable bag were better than the other cleaning methods (P<0.05). The phones which were cleaned by alcohol had the significantly lower ATP value than the others (P<0.05). The daily cleaned phones were much cleaner than the weekly cleaned ones (P<0.001). The onset time of alcohol disinfection was above 10 minutes, the ATP value of 10 minutes and 20 minutes after alcohol disinfection were (271.40±157.76) RLU and (344.30±147.79) RLU, respectively. The disinfection effect of Alcohol met the standard of cleanness (ATP<500RLU). The ATP value were (2129.90±1344.91) RLU and (444.75±228.47) RLU,respectively, after using general wipes or sanitary wipes to clean and disinfect the phones. The cleaning effect of sanitary wipes was better than general wipes (P<0.001), and close to the standard of cleanliness. Conclusion: The mobile phones of medical staff were seriously polluted. Some good health habits, including hands washing before touching the phones, daily cleaning the phones with alcohol or sanitary wipes, and using protective disposable bags, could keep the cleanness of cell phones and decrease the pathogen pollution.