Abstract: Objective: To explore the effectiveness of mix-methods training program on premature infant care of parents.Methods: Self- designed questionnaires were applied to investigate 180 parents of premature infants about care knowledge, skills before and after training when the babies were hospitalized. Results: After training, the caring knowledge and skills on premature infant care of the premature infants' parents improved (P<0.001). Conclusion: The premature infants' parents had obviously improved their knowledge and skills after the mix-methods training program. It could be helpful for their home care after the premature infants discharge.