Abstract: Objective: To explore the relationship between professional self-concept and job satisfaction among the HIV/ AIDS department nurses, and to provide the references for improving HIV/AIDS department nurses' professional self-concept and job satisfaction. Methods: A total of 188 nurses working in the HIV/AIDS department from 6 designated AIDS hospitals in Guangdong province were recruited and investigated by Professional Self-Concept of Nurses Instrument (PSCNI) and McCloskey-Mueller Satisfaction Scale (MMSS). Results: The mean scores of professional self-concept and job satisfaction among HIV/AIDS department nurses were (83.36±10.69) and (94.39±15.54), respectively. Job satisfaction has significantly positive relationship with management ability, professional skills, flexibility and degree of satisfaction of professional self-concept(P<0.01) and had no statistical relationship with the communicating skill of professional self-concept. Conclusion: The HIV/AIDS department nurses have positive professional self-concept and a little bit high job satisfaction. Management ability, professional skills, flexibility, and satisfaction of professional self-concept are the influencing factors of job satisfaction. The hospital should cultivate HIV/AIDS department nurse's positive self-concept to improve their job satisfaction, to give play to nurses' enthusiasm and creativity, and to promote the development of HIV/AIDS care.