Abstract: Objective: To describe the Horizontal Violence (HV) and the team psychological safety climate of nurses in tertiary hospitals and determine their relationships. Methods: A survey was conducted in 187 nurses from 6 departments of 2 tertiary hospitals in Wenzhou, Zhejiang province by using Horizontal Violence Scale and Team Psychological Safety Climate Scale. Results: There were 135 (72.2%) nurses in the study reported the HV, and "Blocking the passage of message" was the highest frequency in HV. The nurses with different professional titles and different staff relations reported HV at different levels (P<0.05). The average score of team psychological safety climate was 60.77±7.15. Those with HV reported lower scores at the total team psychological safety climate and each dimensions than those without HV (P<0.05). Conclusion: The nurses with junior professional title and contract nurses were more likely to report HV, it seems that a good humanistic environment, a suitable HV coping strategy or system could reduce the incidence of HV and improve the team psychological safety of nurses.