Abstract: Objective: To investigate nurses' behavior of patients' privacy protection and its influencing factors. Methods: The convenient sampling method was used, 616 nurses in two tertiary-level hospitals were investigated by the Patient Privacy Scale (PPS). Results: The dimension "favorable environment" got the highest score, while "sexual privacy" got the lowest. The item "when necessary, equipment as folding screen and covering are used to ensure privacy" got the highest score. The item "nobody is allowed to watch the practices unless approved by the patient". The multiple regression analysis showed that age, duty, privacy protect standards in department, and the usage of materials which to protect patients' privacy influenced nurses' behavior of patients' privacy protection (P<0.05). Conclusion: The effect of age and duty should be concerned for nurses' behavior of patients' privacy protection. Standards should be developed to create an ethical environment for patients' privacy protection.