Abstract: Objective: To investigate the status of professional values and practice environment among nurses with master or doctoral degree, and analyze the impact of perceived practice environment on professional values. Methods: Totally 1223 nurses with master or doctoral degree from 115 tertiary hospitals in 22 provinces were investigated with the Nurses Professional Values Scale -Revised (NPVS-R) and the Practice Environment Scale of the Nursing Work Index (PES-NWI). Results: The mean score of professional values was (4.51±0.51). The factor scores from high to low were caring (4.57±0.52), professionalism (4.50±0.60), justice (4.47±0.65), activism (4.42±0.62) and trust (3.63±0.45). The mean score of practice environment was (3.21±0.49). Hierarchical regression showed that nurses with lower initial education (β=-0.097), higher job satisfaction (β=0.174) and higher monthly income (β=0.063) showed higher professional values (R2=0.063). After controlling for general information, high-quality care services base (β=0.371) and nurse-physician co-operation (β=0.104) in nursing work environment were significant influencing factors of professional values, which explained 16.5% of the total variance. Conclusion: Nurses with master or doctoral degree in tertiary hospitals show positive professional values, which are influenced by initial education, job satisfaction, monthly income and practice environment. Nursing managers should provide proper guidance and management to help them establish correct professional values.