Abstract: Objective: To investigate the effects of risk assessment and pulmonary embolism prophylaxis among hospitalized patients, provide a reference for regulating nursing standard to reduce pulmonary embolism occurrence. Methods: Risk assessment and stratified pulmonary embolism prophylaxis strategies were applied to all hospitalized patients. Results: The pulmonary embolism diagnosis rate was improved; risk assessment rate reached 77.50%, targeted prevention strategies utilization rate was 22.71%. There were less pulmonary embolism high-risk patients in internal medicine, and more in surgery department. Patients who received pulmonary embolism prophylaxis had significantly lower prevalence than who did not. Conclusion: Nurses were capable to implement pulmonary embolism risk assessment and identify high-risk patients, while prophylaxis strategies need to be improved. The effects of risk assessment and prophylaxis strategies should be improved by training, quality control and resource allocation.