Abstract: Objective: To comparatively analyze the current status of the prevention of VTE between non-operating departments and operating departments from the tertiary grade A hospitals by multiple center surveys, so as to provide guidance for clinical nursing work to carry out comprehensive prevention of VTE in hospitals. Methods: Using a self-designed questionnaire, we conducted a survey on 5 278 nurses from 29 provinces (municipalities and autonomous regions) at the 2015 Symposium of Chinese Nursing Association of Surgical Nursing. Among them, 3 062 nurses were from operating departments, another 2 216 nurses were from non-operating departments. Results: The standardized prevention procedure and risk assessment for VTE in the operating departments were obviously better than those in the non-operating departments, and the P values were 0.042 and 0.046, respectively. Nurses' overall knowledge level of VTE in operating departments was higher than those in non-operating departments, P=0.001. There was still lack of national standardized procedures of the prevention and nursing for VTE. Conclusion: At present, it is urgent to establish the consensus on the prevention and nursing for VTE in China, and comprehensively and rapidly promote the standardization and homogenization of the clinical prevention and nursing work for VTE, and improve clinical nurses' prevention and control level for VTE at the same time.