Abstract: Objective: To analyze the training mode of aviation medical rescue nurse specialist suitable for Chinese national conditions, and to establish the qualification standards and training standards for the aviation medical rescue specialist. Methods: Retrospectively analyzed the training situation of the aviation medical rescue specialist nurses in our center from September 2010 to September 2016, and summed up the practical experience of the training of the aviation medical rescue specialist in the past 6 years. We made a training plan for aviation medical rescue specialist nurses according to "specialist nursing nurse training program" issued by the National Ministry of Health and the professional training of the specialist nurses at home and abroad, combined with the characteristics of nursing professional and the training of specialist nurses in Beijing. Training content included medical knowledge, knowledge related to aviation flight and comprehensive training. The methods of teaching, situational simulation training, case analysis and clinical practice were used in teaching. Through professional training and examination, in which objective structured clinical examination accounted for 30%, theory examination 15%, operational examination 15%, study report 15%, nursing review writing 15%, training attendance 5% and training performance 5%, above 80 points was qualified and given certification. Re-training was conducted after 5 years of certification, and continuing certification was given if qualified. Results: A total of 30 nurses accepted aeromedical rescue training, and the mean score of examination achievement was 86.7. Through the systematic professional training, nurses' knowledge related to the aviation rescue, specialized knowledge, skills and comprehensive quality were improved significantly. Conclusion: The training of aviation medical care nurse specialist achieved significant effects in our center, which was an effective mode to train aviation medical care nurse specialist.