Abstract: Objective: To test the reliability and validity of the evaluation questionnaire of head nurses leadership. Methods: Totally 440 nurses were recruited from five tertiary level first class hospitals in Hangzhou, Ningbo and Shaoxing. Exploratory factor analysis, Cronbach's α and Correlation coefficient measurement were conducted to test the reliability and validity of questionnaire. Results: The evaluation questionnaire of head nurses leadership contained five factors and 44 items; the cumulative variance contribution rate was 73.444%; Cronbach's α was 0.988; Cronbach's α of 5 factors were 0.837 to 0.978; the correlation coefficient of each index item and total were 0.597 to 0.854; the retest reliability was 0.791. Conclusion: The evaluation questionnaire of head nurses leadership has good reliability and validity and can be used in the evaluation of head nurses leadership.