Abstract: Objective: To investigate the establishment and advanced nursing practice status of Nurse-Led Clinics in tertiary hospitals in China. Method: A convenient sampling of 330 tertiary hospitals was conducted using a self-designed questionnaire, covering 19 provinces (autonomous regions and municipalities) in China. Results: Totally 926 Nurse-Led Clinics were established. The top five clinics were Peripherally Inserted Central Catheter (PICC) care, wound/stoma/incontinence care, diabetes mellitus health education, perinatal care and peritoneal dialysis care. In total, there were 2701 registered nurses working in the Nurse-Led Clinics. Among them, 81.2% had a bachelor or higher degree of nursing. Specialist nurses qualification authentication of health administration departments or nursing academic associations were necessary for clinic nurses, comprised 55.8% and 55.2% of cases, respectively. 61.2% of hospitals had set up the charging expense for diagnosis and treatment in Nurse-Led Clinics. Notably, 82.1% of them emphasize that a lack of supporting policies was the main difficulty when establishing clinics, while 73.6% reported the major barrier to operate Nurse-Led Clinics was a lack of explicit charge standard. Conclusions: Nurse-Led Clinics in China experience a rapid growth. The effectiveness of Nurse-Led Clinics in service delivery and clinical outcomes are expected. It is suggested that relevant regulations and policies should be set up, in order to improve the practice environment and facilitate the sustainable development of Nurse-Led Clinics in China.