Abstract: Objective: To investigate ICU nurses' knowledge, attitudes and practice about patients' sleep status. Methods: Totally 133 ICU nurses from ICU specialist nurse training course were recruited using convenience sampling method and investigated online using self-designed "ICU nurses' knowledge, attitudes and practice about patients' sleep status questionnaire". Results: About 82.0% respondents described they didn't know how to assess patients' sleep status. Nearly 80% nurses had never heard about sleep evaluation tools. Only 12.8% responders had received related training. Over 80% respondents thought ICU nurses have the responsibility to evaluate patients' sleep status every day. Nearly 90% respondents thought the ICU should supply some training program about patient's sleep status evaluation and made related protocols. Less than 40% respondents recorded patients' sleep status and less than 5% respondents described that their units provided some sleeping monitoring equipment. Conclusion: ICU nurses have positive attitude towards managing patients' sleep status, but lack related knowledge and behavior. Nursing managers should develop knowledge's about solving ICU patients' sleeping deprivation and these knowledge should be put into ICU specialist nurse training program, so as to guide ICU nurses' practice to relieve ICU patients' sleeping deprivation and improve patients' sleep quality in ICU.