Abstract: Objective: To assess the effect of acute thrombolysis protocol on "door-to-needle time" (DTN) and improvement in outcomes following acute stroke. Method: A protocol for faster DTN was introduced and nursing process was optimized. We reviewed the outcomes before and after the introduction of the new protocol. Results: After implementing new protocol, DTN was significantly reduced, as well as staffs' satisfaction was improved. Conclusion: Implementation of specific protocols to reduce DTN leads to improve outcomes.