Abstract: Objective: To examine the effects of a nurse stratified training program based on nursing clinical ladder model, provide a reference for nurse stratified training.Method: Based on the core competencies and promotion requirements for each level, the researchers evaluated training needs and therefore determined training contents, then carried out the stratified training program for practice. The outcome measurements included the amount of nurse staff participated training, the pass rate of nurse standardization training examination and the scores of nurse clinical comprehensive ability before and after the implementation. Results: The amount of nurse attending training increased from 1674 in 2012 to 3729 in 2014. The individual annual average frequency of training improved from 2.09 in 2012 to 4.66 in 2014, and the pass rate of nurse standardization training examination improved from 89.56% in 2012 to 97.50% in 2014 (P<0.05), and the scores of nurse clinical comprehensive ability were significantly increased with a mean score from 74.46±7.62 in 2012 to 81.13±8.09 in 2014 (P<0.05). Conclusion: The nurse stratified training program based on nursing clinical ladder model could encourage nurses to participate in training effectively, improve nurses' core competencies, and promote nursing post management, which is an effective method for nurses' on-the-job training.