Abstract: Objective: To construct the standardized nursing system, and to apply it to clinic practice. Methods: We constructed the standard system content, took organization mobilization, carried out learning discussion and hierarchical assessment and acceptance methods, to establish the standardized nursing system hierarchically, gradually and in all directions. Results: The quality of nursing services was improved, a high quality of nursing team was built and the nursing science development was promoted after the clinic practice of the standardized nursing system. Conclusion: Construction and clinic practice of standardized nursing system effectively promote the development of nursing work, which provide an efficient method for the sustainable development of the nursing work in the research-oriented hospital.