Abstract: Objective: To provide reference for hospital to carry out continuing education, this paper has explored the status of knowledge on ICU-acquired weakness in Intensive Care Unit nurses. Methods: Using questionnaire to investigate 470 nurses in three tertiary grade A hospitals in Henan province and taking semi-structured interview method to interview 20 clinical nurses and nursing managers who came from a tertiary comprehensive teaching hospital. Results: The average score of the related knowledge about ICU-acquired weakness in nurses was 32.17±4.39 and score rate was 54.3%. The main reasons causing this phenomenon were lacking of systematically and professional training, single access methods and low attention. Professional title, educational level and working length were the main factors that influenced the knowledge score. Conclusion: In order to reduce the mortality rate, disability rate, the time stay in hospital, hospitalization costs,improve the quality of life and satisfaction of patients, the professional knowledge training is necessary in hospital.