Abstract: The staffs in Central Sterile Supply Department of the First Affiliated Hospital of Nanchang University were divided into five levels to receive sterile and supply theoretical and skill training. The training focused on pecialized position training besides various kinds of training. After two years of training, the staffs'scores improved significantly than those before training, and the examination pass rate is currently 100%. Therefore, layered staff training could optimize training resources,save training costs, improve training efficacy, and comply with the characteristics of sterile supply decipline and regularity of personnel growth.