Abstract: Objective: To investigate the effects and problems of 3 professional standards implementation in Central Sterile Supply Department (CSSD) of the hospitals in Anhui province. Methods: The CSSD professional standards tracing evaluation questionnaire made by Hospital Infection Control Standard Committee of the Ministry of Health was used in the survey of 105 hospitals and the Departments of Public Health from 8 cities and 63 counties. Results: The main implementation of 3 standards by administrative department of health in 8 cities included making provisions on administration in the region, inspecting institutions based on the standards, and accepting it into the accreditation in hospital. They all organized relevant people to attend the provincial training program. Nosocomial infection and CSSD personnel accounted for the highest proportion (>90%), the second was staffs from health administrative sectors, administrative leaders in hospitals and staffs in nursing department (>80%).Sixty two hospitals obtained CSSD acceptance certificate issued by provincial Bureau of Health. Among of them, 98% CSSDs were newly built or expanded after 3 standards announced. But some problems existed, for example, the qualified CSSDs were insufficient to help surrounding medical institutions, which influenced the effects of 3 standards in advancing social management.CSSD centralized management, disposition of exotic instruments and mega-, excess medical devices needed further standard regulations. Conclusion: The CSSD centralized management ability should be expanded. The qualified CSSDs should play important roles in improving disinfection quality of primary medical organizations and preventing hospital infection, as well as promote the thoroughly implementation of 3 CSSD professional standards.