Abstract: Objective: To investigate the training needs of specialist nurses of assisted reproductive technology (ART), and to provide feasible suggestion for the ART nurse training. Methods: We recruited and investigated 121 nurses from five ART centers in Hunan province which had the license from the National Health and Family Planning Commission. Results: Totally 116 (95.87%) of nurses regarded that the training of ART knowledge and related information was very important. Conclusions: The specialized knowledge of specialist nurses in ART should be greatly improved, and the managers should establish targeted training system to cultivate the nurses through various and focused ways.