Abstract: Objective: To investigate the pressure injury prevalence among secondary and tertiary general hospitals in different areas of Guangdong province. Methods: A cross-sectional design was used in this study. Totally 25 264 hospitalized patients from 25 secondary and tertiary general hospitals in different regions of Guangdong province were recruited. There were 267 pressure injury patients among them. The authors used pressure injury questionnaire to describe the source of pressure injury occurrence, location and stage of pressure ulcer. Results: The overall prevalence rates of pressure injury in 25 hospitals ranged from 0 to 3.5%, with an average of 1.3%. When the stage I was excluded, the total prevalence rates range from 0 to 3.1%, with an average of 1.1%. Pressure injury prevalence rates were higher in intensive care unit, geriatrics, respiratory medicine and so on. The most common stages of pressure injuries were stage II and stage III, the most common locations of pressure injuries were sacrum and feet. Conclusion: The overall prevalence of pressure ulcers in 25 hospitals was slightly lower than the rate reported in the results of other studies. The management and report of stage I pressure injury were still inadequate, and the treatment should be more active. Communications between study centers and various clinical departments of the hospital need to be improved.