Abstract: Objective: To identify the status and impact factors of ICU patients' discomfort, and provide references for interventions of reducing ICU patients' discomfort level. Method: Totally 185 patients transferred from ICU in 3 tertiary Grade A hospitals were recruited in this study, the participants were investigated with Chinese version-IPREA questionnaire, Critical-Care Pain Observation Tool, Simplified Acute Physiology Score, Richmond Agitation-Sedation Scale and Disease-related Questionnaire. Results: The mean total score of discomfort scale was (3.35±0.93). Thirst and isolation got the highest scores, and coldness and lack of information got the lowest. Multiple step-wise regression analysis showed that mechanical ventilation, marital status, duration of mechanical ventilation, SAPS II score, physical constraint hours, sedation, accounted for 31.2% of the total variances. Conclusions: ICU patients experience significant levels of discomfort, clinical staff should take timely and appropriate intervention strategies to improve ICU patients' comfort.