Abstract: Objective: To analyze the effect of the application of standardized secondary triage mode in the emergency waiting patients. Method: Totally 4320 patients were assigned to control group by convenient sampling method, and 4543 emergency waiting patients were assigned to observed group. The patients in the control group received routine secondary triage. In contrast, the patients in the observed group received standardized secondary triage. Analyze and compare the waiting patient satisfaction and incidence of accident between two groups. Results: The satisfaction rate on service,humanistic care and overall satisfaction in the observed group were higher than the rates from control group (P<0.05). The incidence of accident of the observed group was lower than the control group (P<0.05). Conclusions: The standardized secondary triage mode provides early identification and intervention to recognize "potential critical patients" in the emergency waiting patients, dynamically adjust the visiting sequence and destination, so that the patients can get timely treatment, achieve the effectiveness and accuracy of secondary triage, protect the safety of medical treatment and improve patient satisfaction.