Abstract: Objective: To investigate and analyze the level of knowledge, attitude, practice to evidence-based nursing and perceived barriers to evidence-based nursing in clinical nurses. Methods: A convenience sample of 375 registered nurses was investigated with demographic data questionnaire, the Evidence-Based Practice Questionnaire, The BARRIERS Scale. Results: The average score of knowledge, attitude, and practice to evidence-based nursing was 4.38±1.02. The score of each dimension from low to high was nursing knowledge and skill (4.26±1.01), nursing practice (4.31±1.32), nursing attitude to evidence-based nursing (4.59±1.24).Multiple stepwise regression analysis indicated that present position, working pressure, occupational attitude, attitude to evidencebased nursing and the quality of research reports entered regression equation, with 27.5% explanation for variance. Conclusion: The level of knowledge, attitude, practice to evidence-based nursing in clinical nurses should be improved, which is affected by nurses' present position, occupational attitude, working pressure, attitude to evidence-based nursing and the quality of research reports.