Abstract: Objective: To formulate allocation standards of nursing human resources by measuring workload of nursing staff in assisted living unit in a long-term care facility. Methods: Based on the results of Delphi expert consultation, convenience sampling method was used in this study. And 28 nursing assistants and 3 registered nurses who worked in assisted living unit of a long-term care facility in Beijing were investigated. Working hour measuring method was used to calculate nursing workload, and human resource allocation formula was used to calculate allocation standard of nursing staff in this unit. Results: For nursing assistants,average daily direct care time of was more than average daily indirect care time (t=13.577, P=0.002), while there was no significant difference between nurses' average daily direct and indirect care time (P>0.05). The actual proportions of nursing assistant and nurse to aged people were 1.120 : 1 and 0.120 : 1 respectively, while the ideal proportions were 0.495 : 1 and 0.070 : 1 by calculation.Conclusion: Nursing workload can be determined scientifically by working hour measuring method, and then allocation standard of nursing staff can be calculated. The proportions of aged people to nursing assistant / nurse in this unit were higher than others.Some countermeasures, including standardizing nursing working process, defining working scope of nursing staff, and reasonably distributing direct and indirect nursing time, could be implemented to decrease the number of nursing staff properly.