Abstract: Objective: To explore the effect of project management on perioperative DVT prevention of patients with spinal fractures. Methods: A historical and comparative method was used. A total of 138 patients with spinal fractures were selected from August 2016 to July 2017 by cluster sampling. Sixty-eight patients were included in the control group from August to December 2016. Seventy patients were included in the observation group from March to July 2017. The control group was treated with regularly thrombus control measures. The observation group was treated with multidisciplinary vascular access project management measures. The incidence of thrombosis, results of laboratory tests, hospital stay and patient satisfaction, the feeling and attitudes toward physician-nurse collaboration were compared between the two groups. Results: The incidence of DVT in the observation group was lower than that in the control group (P<0.05). The hospitalization days of the observation group were fewer than that of the control group, and patients’ satisfaction was higher than the control group. The differences were statistically significant (P<0.05). After the implementing of the multidisciplinary vascular access project management, the attitudes towards physician-nurse collaboration and the degree of cooperation between doctors and nurses were slightly higher, while the differences were not statistically significant. Conclusion: Multidisciplinary vascular access project management has a good effect on the perioperative prevention of DVT in patients with spinal fractures, and it is helpful to reduce the length of hospital stay, improve patient satisfaction, and strengthen physician-nurse collaboration.