Abstract: Objective: To explore the effect of preoperative intravenous supplement 5% GNS 1000mL on stress response and postoperative stress hyperglycemia in gastric cancer patients with general anesthesia during continuous endoscopy surgery. Methods: Totally 122 gastric cancer patients with general anesthesia during continuous endoscopy surgery were randomLy divided into rehydration intervention group and regular group. Rehydration intervention group: we transfused 5%GNS 1000mL by vein for patients who were forbidden to eat and drink over 12 hours. After the intravenous infusion, the puncture trocar was remained and brought into the operating room. Regular group: we did not transfuse any liquid for patients before the continuous surgery. Then we compared the degree of thirsty and hungry, mean arterial pressure, heart rate and changes in blood sugar before surgery between the two groups. Results: Patients' degree of thirsty and hungry before surgery had significant statistical differences (P<0.001). Patients' mean arterial pressure and heart rate before anesthesia in regular group were significantly different from rehydration intervention group (P<0.001). The blood sugar at 24 hours after surgery had a significant rise in both groups, and the blood sugar at 24 hours and 48 hours after surgery had significant statistical differences (P<0.05) between groups. Conclusion: Preoperative intravenous supplement 5% GNS 1000ML can reduce the degree of thirsty and hungry and surgical stress response in gastric cancer patients with continuous endoscopy surgery, and can speed up the recovery of blood sugar after surgery.