Abstract: Objective: To investigate the knowledge of enteral nutrition guidelines among intensive care nurses in a three-level grade A hospital in Beijing. Method: Totally 60 ICU nurses were selected in one three-level grade A hospital using a self-developed questionnaire, which includes the patients' general condition and an enteral nutrition guidelines related knowledge questionnaire. Results: The average score of enteral nutrition guidelines related knowledge questionnaire was 16.9±2.3, accounting for 73.68% of the total score (22 points), which is in the middle level; the main influence factor of ICU nurses' knowledge was the level of education. Conclusions: The nurses' knowledge of enteral nutrition guidelines were in the middle level, the areas of residual gastric volume assessing, aseptic principles of nasal feeding and new technology should be strengthened.