Abstract: Objective: To understand the current status of the layout of the building, equipment, cleaning, disinfection and management of the Central Sterile Supply Department (CSSD) in the secondary above hospitals of Xinjiang. Methods: The authors carried out a self-designed questionnaire survey about current situation of CSSD management in 104 secondary or above medical institutions of Xinjiang. Results: Among them, 50 CSSDs were centralized supply, 47 hospitals managed operation instruments in CSSD, and 19 hospitals managed oral apparatus in CSSD. There were separate buildings for CSSD in 51 hospitals.There were clearly differentiated areas in 65 hospitals, and buffer regionals in 62 institutions. Neither cross-contamination nor countercurrent was found in 82 hospitals. Totally 56 hospitals equipped with automatic washing machine, bandage or cotton rope were used for packing in 34 hospitals. Conclusion: There were several problems, including decentralized management,unreasonable building facilities, insufficient and simple equipment, in some CSSD of the secondary or above medical hospitals in Xinjiang. As a result, non-standard cleaning, disinfection and sterilization probably produced potential safety hazard to patients.