Abstract: Objective: To develop a Knowledge, Attitude, and Practice (KAP) questionnaire for Catheter-Related Thrombosis prevention and treatment among nursing graduate students and to test its reliability and validity, to provide a basis for targeted prevention and control training. Methods: Based on the KAP theory, the questionnaire was developed through literature analysis, expert panel discussions, expert consultations, and pilot surveys. In June and July 2023, a convenience sampling method was used to select 363 and 384 nursing graduate students in the clinical practice phase from different tertiary general hospitals in Hunan province as research subjects. Item analysis, exploratory factor analysis, confirmatory factor analysis, and reliability testing were conducted. Results: The final questionnaire consists of 3 dimensions with a total of 55 items. The Kendall's coefficient of concordance for the two rounds was 0.175 and 0.252 (P<0.05). Exploratory factor analysis extracted 9 common factors, with the cumulative variance contribution rate of the three dimensions of knowledge, attitude, and practice ranging from 52.2% to 88.3%. Confirmatory factor analysis showed a good model fit, χ2/df=2.360, RMSEA=0.056, GFI=0.914, NFI=0.856, CFI=0.902, IFI=0.903, the AVE values for each dimension ranged from 0.536 to 0.824. The average content validity index was 0.913, and the item content validity index ranged from 0.800 to 1.000. The total Cronbach's α coefficient of the questionnaire was 0.944, the split-half reliability was 0.960, the test-retest reliability was 0.933, Conclusion: The KAP questionnaire for Catheter-Related Thrombosis prevention and treatment among nursing graduate students has good reliability and validity and is suitable for assessing the nursing graduate students Catheter-Related Thrombosis prevention and treatment.

Key words: nursing postgraduate; Catheter-Related Thrombosis; knowledge-attitude-practice; reliability; validity